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Methane production was measured following the replacement of corn with DDGS in vitro and also after the simultaneous replacement of corn and corn oil with DDGS (for 30% of the diet) in vivo. In vitro substitution of corn with DDGS increased the amount of methane produced per milligram of DM digested. Likewise, in vivo methane production was increased by 44% when corn and corn oil were replaced with DDGS. The greater energy value of DDGS relative to corn in a concentrate-based diet is not due to decreased methanogenesis.