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Crossbred yearlings (n = 90) were allotted to one of ten diets containing 0%, 20% and 40% wet distillers grains (WDG) with or without vitamin E supplementation and distillers solubles. Strip loin and tenderloin steaks were obtained and tested for their fatty acid profiles using gas chromatography. WDG diets increased linearly (P < 0.05) the polyunsaturated fatty acids (PUFA) containing 18 or more carbons and trans fatty acids in both muscles. No significant differences were found for total saturated and unsaturated fatty acids. Dietary inclusion of neither vitamin E nor distiller solubles significantly changed PUFA, trans, omega-6 or omega-3 fats in strip loins and tenderloins. Therefore, changes in the fat¬ty acid profile of beef are a consequence of WDG, not the solubles or vitamin E.