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A two-year study evaluated feeding dried distillers grains (DDG) during heifer development on growth and reproductive performance. Supplements provided similar CP, energy, lipid, and fatty acids. Protein degradability of the supplements differed such that undegradable intake protein exceeded requirements of DDG heifers. Heifer pubertal development, artificial insemination (AI) pregnancy rate, and overall pregnancy rate were not affected by supplement. However, AI conception rate and AI pregnancy rate were improved by feeding DDG in the heifer development diet.