Date of this Version
THE JOURNAL OF BIOLOGICAL CHEMISTRY VOL. 290, NO. 4, pp. 2225–2234, January 23, 2015
Background: PRODH and P5CDH from Thermus thermophilus are monofunctional enzymes in proline catabolism.
Results: Steady-state kinetics and intermediate trapping data show the PRODH and P5CDH reactions are coupled by a channeling step.
Conclusion: Substrate channeling in monofunctional enzymes is achieved via weak interactions.
Significance: Evidence for substrate channeling between monofunctional proline catabolic enzymes is shown and confirms the Rosetta Stone hypothesis.