J. Biol. Chem. 2011, 286:43144-43153.
doi: 10.1074/jbc.M111.292474 originally published online October 19, 2011
Background: Trifunctional proline utilization A (PutA) proteins are multifunctional flavoproteins that catalyze two reactions and repress transcription of the put regulon.
Results: PutA from Escherichia coli is a V-shaped dimer, with the DNA-binding domain mediating dimerization.
Conclusion: Oligomeric state and quaternary structures are not conserved by PutAs.
Significance: The first three-dimensional structural information for any trifunctional PutA is reported.