Atomic resolution experimental phase information reveals extensive disorder and bound 2-methyl-2,4-pentanediol in Ca²⁺-calmodulin

Authors

Jiusheng Lin, University of Nebraska-LincolnFollow
Henry van den Bedem, Stanford University
Axel T. Brunger, Stanford University
Mark A. Wilson, University of Nebraska-LincolnFollow

Document Type

Article

Date of this Version

2016

Citation

Acta Cryst. (2016). D72, 83–92

http://dx.doi.org/10.1107/S2059798315021609

Comments

Copyright 2016 International Union of Crystallography

Abstract

Calmodulin (CaM) is the primary calcium signaling protein in eukaryotes and has been extensively studied using various biophysical techniques. Prior crystal structures have noted the presence of ambiguous electron density in both hydrophobic binding pockets of Ca²⁺-CaM, but no assignment of these features has been made. In addition, Ca²⁺-CaM samples many conformational substates in the crystal and accurately modeling the full range of this functionally important disorder is challenging. In order to characterize these features in a minimally biased manner, a 1.0 A resolution single-wavelength anomalous diffraction data set was measured for selenomethionine-substituted Ca²⁺-CaM. Density-modified electron-density maps enabled the accurate assignment of Ca²⁺-CaM main-chain and side-chain disorder. These experimental maps also substantiate complex disorder models that were automatically built using lowcontour features of model-phased electron density. Furthermore, experimental electron-density maps reveal that 2-methyl-2,4-pentanediol (MPD) is present in the C-terminal domain, mediates a lattice contact between N-terminal domains and may occupy the N-terminal binding pocket. The majority of the crystal structures of target-free Ca²⁺-CaM have been derived from crystals grown using MPD as a precipitant, and thus MPD is likely to be bound in functionally critical regions of Ca²⁺-CaM in most of these structures. The adventitious binding of MPD helps to explain differences between the Ca²⁺-CaM crystal and solution structures and is likely to favor more open conformations of the EF-hands in the crystal.