Surface Display of Recombinant Proteins on Bacillus thuringiensis Spores

Authors

Cheng Du, University of Nebraska Medical Center, Omaha, Nebraska
Wing C. Chan, University of Nebraska Medical Center, Omaha, Nebraska
Timothy W. McKeithan, University of Nebraska Medical Center, Omaha, Nebraska
Kenneth W. Nickerson, University of Nebraska-LincolnFollow

Date of this Version

June 2005

Comments

Published in APPLIED AND ENVIRONMENTAL MICROBIOLOGY, June 2005, p. 3337–3341 Vol. 71, No. 6. Copyright © 2005, American Society for Microbiology. Used by permission.

Abstract

The insecticidal protoxin from Bacillus thuringiensis has been shown to be a major component of the spore coat. We have developed a novel surface display system using B. thuringiensis spores in which the N-terminal portion of the protoxin is replaced with a heterologous protein. The expression vector with a sporulationspecific promoter was successfully used to display green fluorescent protein and a single-chain antibody (scFv) gene that encodes anti-4-ethoxymethylene-2-phenyl-2-oxazolin-5-one (anti-phOx) antibody. The spores that carry the anti-phOx antibody can bind to phOx specifically.