Long-term activity of biohybrid coatings of atrazine-degrading bacteria Pseudomonas sp. ADP

Authors

Angela K. Pannier, University of Nebraska-LincolnFollow
T. Lehrer, Technion, Israel Institute of Technology, Technion city, Faculty of Civil and Environmental Engineering, Haifa 32000, Israel
M. Vogel, Helmholtz-Zentrum Dresden-Rossendorf, Institute of Resource Ecology, Bautzner Landstrasse 400, D-01328 Dresden, Germany
U. Soltmann, GMBU e.V., Department of Functional Coatings, Bautzner Landstrasse 45, D-01317 Dresden, Germany.
H. Bottcher, GMBU e.V., Department of Functional Coatings, Bautzner Landstrasse 45, D-01317 Dresden, Germany.
S. Tarre, Technion, Israel Institute of Technology, Technion city, Faculty of Civil and Environmental Engineering, Haifa 32000, Israel
M. Green, Technion, Israel Institute of Technology, Technion city, Faculty of Civil and Environmental Engineering, Haifa 32000, Israel
J. Raff, Helmholtz-Zentrum Dresden-Rossendorf, Institute of Resource Ecology, Bautzner Landstrasse 400, D-01328 Dresden, Germany
K. Pollmann, Helmholtz-Zentrum Dresden-Rossendorf, Helmholtz Institute Freiberg for Resource Technology, Bautzner Landstrasse 400, D-01328 Dresden, Germany

Date of this Version

2014

Citation

RSC Adv., 2014, 4, 19970

Comments

This journal is © The Royal Society of Chemistry 2014. Used by permission.

Abstract

The atrazine-degrading bacterial strain Pseudomonas sp. ADP was immobilized by the sol–gel process within thin silica layers coated onto water-retaining carrier materials (expanded clay pellets and scoria). The performance of the obtained biohybrid material has been investigated concerning long-term activity under non-growth conditions. Experiments were run in phosphate buffer containing atrazine (20 mg l^-1) as the sole source of carbon and nitrogen. Even after one year of consecutive batch tests, P. ADP immobilized onto expanded clay pellets showed a high atrazine degradation activity. In the course of long-term batch experiments, the average amount of removed atrazine was about 94% during each assay cycle. Staining with CTC revealed that in spite of cultivation under non-growth conditions over a period of one year, immobilized cells were still vital and showed respiratory activity.