Long-term activity of biohybrid coatings of atrazine-degrading bacteria Pseudomonas sp. ADP

Authors

• Angela K. Pannier, University of Nebraska-LincolnFollow
• T. Lehrer, Technion, Israel Institute of Technology, Technion city, Faculty of Civil and Environmental Engineering, Haifa 32000, Israel
• M. Vogel, Helmholtz-Zentrum Dresden-Rossendorf, Institute of Resource Ecology, Bautzner Landstrasse 400, D-01328 Dresden, Germany
• U. Soltmann, GMBU e.V., Department of Functional Coatings, Bautzner Landstrasse 45, D-01317 Dresden, Germany.
• H. Bottcher, GMBU e.V., Department of Functional Coatings, Bautzner Landstrasse 45, D-01317 Dresden, Germany.
• S. Tarre, Technion, Israel Institute of Technology, Technion city, Faculty of Civil and Environmental Engineering, Haifa 32000, Israel
• M. Green, Technion, Israel Institute of Technology, Technion city, Faculty of Civil and Environmental Engineering, Haifa 32000, Israel
• J. Raff, Helmholtz-Zentrum Dresden-Rossendorf, Institute of Resource Ecology, Bautzner Landstrasse 400, D-01328 Dresden, Germany
• K. Pollmann, Helmholtz-Zentrum Dresden-Rossendorf, Helmholtz Institute Freiberg for Resource Technology, Bautzner Landstrasse 400, D-01328 Dresden, Germany

Document Type

Article

Date of this Version

2014

Citation

RSC Adv., 2014, 4, 19970

Comments

This journal is © The Royal Society of Chemistry 2014. Used by permission.

Abstract

The atrazine-degrading bacterial strain Pseudomonas sp. ADP was immobilized by the sol–gel process within thin silica layers coated onto water-retaining carrier materials (expanded clay pellets and scoria). The performance of the obtained biohybrid material has been investigated concerning long-term activity under non-growth conditions. Experiments were run in phosphate buffer containing atrazine (20 mg l^-1) as the sole source of carbon and nitrogen. Even after one year of consecutive batch tests, P. ADP immobilized onto expanded clay pellets showed a high atrazine degradation activity. In the course of long-term batch experiments, the average amount of removed atrazine was about 94% during each assay cycle. Staining with CTC revealed that in spite of cultivation under non-growth conditions over a period of one year, immobilized cells were still vital and showed respiratory activity.