Our laboratory conducts research at the interface of chemistry and biology. We are actively developing strategies for labeling proteins with fluorescent molecules as well as controlling biological processes with precise spatial and temporal resolution. These efforts are supported by a combination of multidisciplinary approaches including protein evolution, synthesis and molecular biology. We are particularly interested in applications pertaining to cell motility and signaling. Towards these goals we are: 1) Developing turn-on fluorescence labels for interrogating protein dynamics, 2) Using split-protein reassembly to control cellular function and 3) Engineering inherently fluorescent proteins.

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2011

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A turn-on split-luciferase sensor for the direct detection of poly(ADP-ribose) as a marker for DNA repair and cell death, Jennifer L. Furman, Pui-Wing Mok, Shengyi Shen, Cliff I. Stains, and Indraneel Ghosh

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A p38α-Selective Chemosensor for use in Unfractionated Cell Lysates, Cliff I. Stains, Elvedin Luković, and Barbara Imperiali

2010

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Toward a General Approach for RNA-Templated Hierarchical Assembly of Split-Proteins, Jennifer L. Furman, Ahmed H. Badran, Oluyomi Ajulo, Jason R. Porter, Cliff I. Stains, David J. Segal, and Indraneel Ghosh

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A General Approach for Receptor and Antibody-Targeted Detection of Native Proteins Utilizing Split-Luciferase Reassembly, Cliff I. Stains, Jennifer L. Furman, Jason R. Porter, Srivats Rajagopal, Richard T. Wyatt, and Indraneel Ghosh

2008

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A General and Rapid Cell-Free Approach for the Interrogation of Protein–Protein, Protein–DNA, and Protein–RNA Interactions and their Antagonists Utilizing Split-Protein Reporters, Jason R. Porter, Cliff I. Stains, Benjamin W. Jester, and Indraneel Ghosh

2007

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Molecules that Target beta-Amyloid, Cliff I. Stains, Kalyani Mondal, and Indraneel Ghosh

2006

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Direct detection of double-stranded DNA: molecular methods and applications for DNA diagnostics, Indraneel Ghosh, Cliff I. Stains, Aik T. Ooi, and David J. Segal

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Sequence-Enabled Reassembly (SEER) Peptides for the Detection of DNA Sequences, Aik T. Ooi, Cliff I. Stains, Jason R. Porter, Indraneel Ghosh, and David J. Segal