A turn-on split-luciferase sensor for the direct detection of poly(ADP-ribose) as a marker for DNA repair and cell death

Authors

Jennifer L. Furman, University of Arizona
Pui-Wing Mok, University of Arizona
Shengyi Shen, University of Arizona
Cliff I. Stains, University of Nebraska-LincolnFollow
Indraneel Ghosh, University of ArizonaFollow

ORCID IDs

Cliff I. Stains

Date of this Version

2011

Comments

Chem. Commun., 2011, 47, 397–399; DOI: 10.1039/c0cc02229b Copyright (c) 2011 The Royal Society of Chemistry. Used by permission.

Abstract

Designed sensors comprising split-firefly luciferase conjugated to tandem poly(ADP-ribose) binding domains allow for the direct solution phase detection of picogram quantities of PAR and for monitoring temporal changes in poly(ADP-ribosyl)ation events in mammalian cells.

Includes Supplementary Material.