Quantitative trait locus mapping and functional genomics of an organophosphate resistance trait in the western corn rootworm, Diabrotica virgifera virgifera

Authors

B. S. Coates, USDA-ARS, Corn Insects & Crop Genetics Research Unit, Ames, IAFollow
A. P. Alves, University of Nebraska-Lincoln
H. Wang, University of Nebraska-Lincoln
X. Zhou, University of Nebraska-Lincoln
T. Nowatzki, University of Nebraska-Lincoln
H. Chen, University of Nebraska-Lincoln
M. Rangasamy, University of Nebraska-Lincoln
H. M. Robertson, University of Illinois, Champaign-Urbana, IL
C. W. Whitfield, University of Illinois, Champaign-Urbana, IL
K. K. Walden, University of Illinois, Champaign-Urbana, IL
Stephen D. Kachman, University of Nebraska-LincolnFollow
B. W. French, USDA-ARS, North-Central Agricultural Research Laboratory, Brooking, SDFollow
L. J. Meinke, University of Nebraska-LincolnFollow
D. Hawthorne, University of Maryland, College Park, MD
C. A. Abel, Iowa State UniversityFollow
T. W. Sappington, Iowa State University
B. D. Siegfried, University of Nebraska-LincolnFollow
N. J. Miller, University of Nebraska-LincolnFollow

ORCID IDs

Stephen D. Kachman

N. J. Miller

Document Type

Article

Date of this Version

2015

Citation

Insect Molecular Biology (2016) 25(1), 1–15

Comments

Published 2015. This article is a U.S. Government work and is in the public domain in the USA

Abstract

The western corn rootworm, Diabrotica virgifera virgifera, is an insect pest of corn and population suppression with chemical insecticides is an important management tool. Traits conferring organophosphate insecticide resistance have increased in frequency amongst D. v. virgifera populations, resulting in the reduced efficacy in many corn-growing regions of the USA. We used comparative functional genomic and quantitative trait locus (QTL) mapping approaches to investigate the genetic basis of D. v. virgifera resistance to the organophosphate methyl-parathion. RNA from adult methyl-parathion resistant and susceptible adults was hybridized to 8331 microarray probes. The results predicted that 11 transcripts were significantly up-regulated in resistant phenotypes, with the most significant (fold increases > 2.43) being an α-esterase-like transcript. Differential expression was validated only for the α-esterase (ST020027A20C03), with 11- to 13-fold greater expression in methyl-parathion resistant adults (P