Genetic analysis of seed traits in Sorghum bicolor that affect the human gut microbiome

Authors

Quinnan Yang, University of Nebraska - LincolnFollow
Mallory J. Suhr Van Haute, University of Nebraska-Lincoln and University of Nebraska at OmahaFollow
Nate Korth, University of Nebraska - LincolnFollow
Scott E. Sattler, University of Nebraska - Lincoln, USDA-ARSFollow
John Toy, University of Nebraska - Lincoln, USDA-ARS
James C. Schnable, University of Nebraska-LincolnFollow
Andrew K. Benson, University of Nebraska - LincolnFollow

Date of this Version

9-26-2022

Citation

Nature Communications | ( 2022) 13:5641. https://doi.org/10.1038/s41467-022-33419-1

Comments

Open access

Includes corrections. Erratum attached below

Abstract

Prebiotic fibers, polyphenols and other molecular components of food crops significantly affect the composition and function of the human gut microbiome and human health. The abundance of these, frequently uncharacterized, microbiome-active components vary within individual crop species. Here, we employ high throughput in vitro fermentations of pre-digested grain using a human microbiome to identify segregating genetic loci in a food crop, sorghum, that alter the composition and function of human gut microbes. Evaluating grain produced by 294 sorghum recombinant inbreds identifies 10 loci in the sorghum genome associated with variation in the abundance of microbial taxa and/or microbial metabolites. Two loci co-localize with sorghum genes regulating the biosynthesis of condensed tannins. We validate that condensed tannins stimulate the growth of microbes associated with these two loci. Our work illustrates the potential for genetic analysis to systematically discover and characterize molecular components of food crops that influence the human gut microbiome.