Scientists at the Roman L. Hruska U.S. Meat Animal Research Center (USMARC) develop new technology in order to increase the efficiency of livestock production and benefit consumers. The USMARC was authorized by Congress on June 16, 1964, and development began in the spring of 1966 on 35,000 acres near Clay Center, Nebraska. Presently, research programs are using a female breeding population of 6,500 cattle of 18 breeds, 4,000 sheep of 10 breeds, and 700 swine litters per year. The USMARC is administered by the Agricultural Research Service (ARS) within the United States Department of Agriculture (USDA). On October 10, 1978, President Carter signed into law a bill which renamed the research facility after former Senator Roman L. Hruska of Nebraska.

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2012

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Dual – Species Biofilm Formation by Shiga – toxin Producing Escherichia coli O157:H7 and O26:H11 serotypes, Rong Wang, Norasak Kalchayanand, John W. Schmidt, and Joseph M. Bosilevac

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The physiologic state of Escherichia coli O157:H7 does not affect its detection in two commercial real-time PCR-based tests, Rong Wang, John W. Schmidt, Terrance M. Arthur, and Joseph M. Bosilevac

2011

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Birth and weaning traits in crossbred cattle from Hereford, Angus, Brahman, Boran, Tuli, and Belgian Blue sires, E. Casas, R. M. Thallman, and L. V. Cundiff

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Energy and Nutrient Recovery fromCattle Feedlots, Roger A. Eigenberg, Bryan L. Woodbury, Brent Auvermann, and Mindy J. Spiehs

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Assessment of pubertal development of boars derived from ultrasonographic determination of testicular diameter, J.J. Ford and T.H. Wise

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Growth curves of crossbred cows sired by Hereford, Angus, Belgian Blue, Brahman, Boran, and Tuli bulls, and the fraction of mature body weight and height at puberty, Harvey C. Freetly, L. A. Kuehn, and L. V. Cundiff

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Determinants of bovine thermal response to heat and solar radiation exposures in a field environment, Brad Scharf, Michael J. Leonard, Robert L. Weaber, Terry L. Mader, G. Leroy Hahn, and Donald E. Spiers

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EFFECT OF FEEDING WET DISTILLERS GRAINS WITH SOLUBLES TO BEEF CATTLE ON AIR AND MANURE QUALITY, M. J. Spiehs, D. N. Miller, B. L. Woodbury, R. A. Eigenberg, V. H. Varel, and D. B. Parker

2010

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Super Shedding of Escherichia coli O157:H7 by Cattle and the Impact on Beef Carcass Contamination, Terrance M. Arthur, Dayna M. Brichta-Harhay, Joseph M. Bosilevac, Norasak Kalchayanand, Steven D. Shackelford, Tommy L. Wheeler, and Mohammad Koohmaraie

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Postweaning growth and carcass traits in crossbred cattle from Hereford, Angus, Brangus, Beefmaster, Bonsmara, and Romosinuano maternal grandsires, E. Casas, R. M. Thallman, L. A. Kuehn, and L. V. Cundiff

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Prediction of genetic values for feed intake from individual body weight gain and total feed intake of the pen, A. J. Cooper, C. L. Ferrell, L. V. Cundiff, and L. D. Van Vleck

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Tenderness – An Enzymatic View, Caroline M. Kemp, Paul L. Sensky, Ronald G. Bardsley, Peter J. Buttery, and Tim Parr

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PRNP Haplotype Associated with Classical BSE Incidence in European Holstein Cattle, Brenda M. Murdoch, Michael L. Clawson, Samuel Yue, Urmila Basu, Stephanie McKay, Matthew Settles, Rossana Capoferri, William W. Laegreid, John L. Williams, and Stephen S. Moore

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REVIEW: Preweaning, Postweaning, and Carcass Trait Comparisons for Progeny Sired by Subtropically Adapted Beef Sire Breeds at Various US Locations, F. A. Thrift, J. O. Sanders, M. A. Brown, A. H. Brown Jr, A. D. Herring, D. G. Riley, S. M. DeRouen, J. W. Holloway, W. E. Wyatt, R. C. Vann, C. C. Chase Jr., D. E. Franke, L. V. Cundiff, and J. F. Baker

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Proportion of the Litter Farrowed, Litter Size, and Progesterone and Estradiol Effects on Piglet Birth Intervals and Stillbirths, J. L. Vallet, J. R. Miles, T. M. Brown-Brandl, and J. A. Nienaber

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Characterization of biological types of cattle (Cycle VIII): Carcass, yield, and longissimus palatability traits, T. L. Wheeler, L. V. Cundiff, S. D. Shackelford, and M. Koohmaraie

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Repellency of a Wax-Based Catnip-Oil Formulation against Stable Flies, Junwei J. Zhu, Christopher A. Dunlap, Robert W. Behle, Dennis R. Berkebile, and Brian Wienhold

2009

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A Genome Wide Survey of SNP Variation Reveals the Genetic Structure of Sheep Breeds, James W. Kijas, David Townly, Brian P. Dalrymple, Michael P. Heaton, Jillian F. Maddox, Annette McGrath, Peter Wilson, Roxann G. Ingersoll, Russell McCulloch, Sean McWilliam, Dave Tang, John McEwan, Noelle Cockett, V. Hutton Oddy, Frank W. Nicholas, Herman Raadsma, and International Sheep Genomics Consortium

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Development and Characterization of a High Density SNP Genotyping Assay for Cattle, Lakshmi K. Matukumalli, Cynthia T. Lawley, Robert D. Schnabel, Jeremy F. Taylor, Mark F. Allan, Michael P. Heaton, Jeff O'Connell, Stephen S. Moore, Timothy P. L. Smith, Tad S. Sonstegard, and Curtis P. Van Tassell

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A multiway analysis for identifying high integrity bovine BACs, Abhirami Ratnakumar, Wesley Barris, Sean McWilliam, Rudiger Brauning, John C. McEwan, Warren M. Snelling, and Brian P. Dalrymple

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Electromagnetic Induction Sensor Data to Identify Areas of Manure Accumulation on a Feedlot Surface, Bryan Woodbury, Scott M. Lesch, Roger A. Eigenberg, Daniel N. Miller, and Mindy J. Spiehs

2008

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Enumeration of Salmonella from Poultry Carcass Rinses via Direct Plating Methods, D. M. Brichta-Harhay, T. M. Arthur, and M. Koohmaraie

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Association of a Bovine Prion Gene Haplotype with Atypical BSE, Michael L. Clawson, Juergen A. Richt, Thierry Baron, Anne-Gaëlle Biacabe, Stefanie Czub, Michael P. Heaton, Timothy P. L. Smith, and William W. Laegreid

2007

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Association of Escherichia coli O157:H7 tir polymorphisms with human infection, James L. Bono, James E. Keen, Michael L. Clawson, Lisa M. Durso, Michael P. Heaton, and William W. Laegreid

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Enumeration of Salmonella and Escherichia coli O157:H7 in Ground Beef, Cattle Carcass, Hide and Faecal Samples Using Direct Plating Methods, D. M. Brichta-Harhay, T. M. Arthur, J. M. Bosilevac, M. N. Guerini, N. Kalchayanand, and M. Koohmaraie

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Comparative Response of Rams and Bulls to Long-Term Treatment with Gonadotropin-Releasing Hormone Analogs, H. Jiménez-Severiano, M. J. D'Occhio, D. D. Lunstra, M. L. Mussard, T. L. Davis, W. J. Enright, and J. E. Kinder

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Placental Expression of the Membrane Form of Folate Binding Protein During Pregnancy in Swine, J. G. Kim and J. L. Vallet

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Interventions to Reduce/Eliminate Escherichia coli O157:H7 in Ground Beef, M. Koohmaraie, T. M. Arthur, J. M. Bosilevac, D. M. Brichta-Harhay, N. Kalchayanand, S. D. Shackelford, and T. L. Wheeler

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Fine-mapping of a QTL influencing pork tenderness on porcine chromosome 2, Stacey N. Meyers, Sandra L. Rodriguez-Zas, and Jonathan E. Beever

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Computing numerator relationships between any pair of animals, L. Dale Van Vleck

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Comparison of Meat Composition from Offspring of Cloned and Conventionally Produced Boars, S. C. Walker, R. K. Christenson, R. P. Ruiz, D. E. Reeves, S. L. Pratt, F. Arenivas, N. E. Williams, B. L. Bruner, and I. A. Polejaeva

2006

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Postweaning growth and carcass traits in crossbred cattle from Hereford, Angus, Norwegian Red, Swedish Red and White, Friesian, and Wagyu maternal grandsires, E. Casas and L. V. Cundiff

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Prion gene haplotypes of U.S. cattle, Michael L. Clawson, Michael P. Heaton, John W. Keele, Timothy P. L. Smith, Gregory P. Harhay, and William W. Laegreid

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Quantification of Calpastatin Using an Optical Surface Plasmon Resonance Biosensor, G. H. Geesink, J. G. P. van der Palen, M. P. Kent, E. Veiseth, G. Hemke, and M. Koohmaraie

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Linkage disequilibrium across six prion gene regions spanning 20 kbp in U.S. sheep, Benedict T. Green, Michael P. Heaton, Michael L. Clawson, and William W. Laegreid

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Lack of Effect of Metyrapone and Exogenous Cortisol on Early Porcine Conceptus Development, H. G. Klemcke, J. L. Vallet, and R. K. Christenson

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Contribution of Postmortem Muscle Biochemistry to the Delivery of Consistent Meat Quality with Particular Focus on the Calpain System, M. Koohmaraie and G. H. Geesink

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Effects of a Single Foot Rot Incident on Weight Performance of Feedlot Steers, G. K. TIBBETTS, T. M. DEVIN, D. GRIFFIN, J. E. KEEN, and G. P. RUPP

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Germplasm Evaluation Program Progress Report No. 23, T. L. Wheeler, Larry V. Cundiff, L. Dale Van Vleck, G.D. Snowder, R.M. Thallman, Steven D. Shackelford, and M. Koohmaraie

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Using simulation models to predict feed intake: Phenotypic and genetic relationships between observed and predicted values in cattle, C. B. Williams, G. L. Bennett, T. G. Jenkins, L. V. Cundiff, and C. L. Ferrell

2005

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Analyses of thermoregulatory responses of feeder cattle exposed to simulated heat waves, T. M. Brown-Brandl, R. A. Eigenberg, G. L. Hahn, J. A. Nienaber, T. L. Mader, D. E. Spiers, and A. M. Parkhurst

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Targeted oligonucleotide-mediated microsatellite identification (TOMMI) from large-insert library clones, Kefei Chen, Christoph Knorr, Kirsten Bornemann-Kolatzki, Jun Ren, Lusheng Huang, Gary A. Rohrer, and Bertram Brenig

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Genetic Relationships Between Male and Female Reproductive Traits in Beef Cattle, G. Gargantini, L. V. Cundiff, D. D. Lunstra, and L. D. Van Vleck

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Perspectives for artificial insemination and genomics to improve global swine populations, Roger J. Gerrits, Joan K. Lunney, Lawrence A. Johnson, Vernon G. Pursel, Robert R. Kraeling, Gary A. Rohrer, and John R. Dobrinsky

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Evidence for a Role for Anti-Müllerian Hormone in the Suppression of Follicle Activation in Mouse Ovaries and Bovine Ovarian Cortex Grafted Beneath the Chick Chorioallantoic Membrane, I. Gigli, R. A. Cushman, C. M. Wahl, and J. E. Fortune

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Characterization of 954 bovine full-CDS cDNA sequences, Gregory P. Harhay, Tad S. Sonstegard, John W. Keele, Michael P. Heaton, Michael L. Clawson, Warren M. Snelling, Ralph T. Wiedmann, Curt P. Van Tassell, and Timothy P. L. Smith

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Post-Harvest Interventions to Reduce/Eliminate Pathogens in Beef, M. Koohmaraie, T. M. Arthur, J. M. Bosilevac, M. Guerini, S. D. Shackelford, and T. L. Wheeler

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Different Levels of Ovine Interferon-t Gene Expressions Are Regulated Through the Short Promoter Region Including Ets-2 Binding Site, Fuko Matsuda-Minehata, Momoko Katsumura, Sho Kijima, Ronald K. Christensen, and Kazuhiko Imakawa

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On-line Classification of US Select Beef Carcasses for Longissimus Tenderness Using Visible and Near-Infrared Reflectance Spectroscopy, S. D. Shackelford, T. L. Wheeler, and M. Koohmaraie

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Allelic Variation in the Erythropoietin Receptor Gene is Associated with Uterine Capacity and Litter Size in Swine, J. L. Vallet, B. A. Freaking, K. A. Leymaster, and R. K. Christenson

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Allelic variation in the erythropoietin receptor gene is associated with uterine capacity and litter size in swine, J. L. Vallet, B. A. Freking, K. A. Leymaster, and R. K. Christenson

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Characterization of biological types of cattle (Cycle VII): Carcass, yield, and longissimus palatability traits, T. L. Wheeler, L. V. Cundiff, S. D. Shackelford, and M. Koohmaraie

2004

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Eugenol Stimulates Lactate Accumulation Yet Inhibits Volatile Fatty Acid Production and Eliminates Coliform Bacteria in Cattle and Swine Waste, V. H. Barel and D. L. Miller

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Association of myostatin on early calf mortality, growth, and carcass composition traits in crossbred cattle, E. Casas, G. L. Bennett, T.P.L. Smith, and L. V. Cundiff

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Beta-2-microglobulin haplotypes in U.S. beef cattle and association with failure of passive transfer in newborn calves, Michael L. Clawson, Michael P. Heaton, Carol G. Chitko-McKown, James M. Fox, Timothy P. L. Smith, Warren M. Snelling, John W. Keele, and William W. Laegreid

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Germplasm Evaluation Program Progress Report No. 22, Larry V. Cundiff, T. L. Wheeler, Keith Gregory, Steven D. Shackelford, M. Koohmaraie, R.M. Thallman, G.D. Snowder, and L. Dale Van Vleck

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Breed Comparisons of Weight, Weight Adjusted for Condition Score, Height, and Condition Score of Beef Cows, Department of Animal Science, University of Nebraska, Lincoln; L. V. Cundiff; and L. D. Van Vleck

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Comparison of Longhorn- and Red Poll-Sired Calves from Crossbred Dams Calving at Two or Three Years of Age, X H. Liu, A Perez Marquez, L. V. Cundiff, and L. D. Van Vleck

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Apoptosis and porcine reproductive and respiratory syndrome virus, Laura C. Miller and James M. Fox

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Interferon type I response in porcine reproductive and respiratory syndrome virus-infected MARC-145 cells, L. C. Miller, W. W. Laegried, J. L. Bono, C. G. Chitko-McKown, and J. M. Fox

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Development of Optimal Protocol for Visible and Near-Infrared Reflectance Spectroscopic Evaluation of Meat Quality, S. D. Shackelford, T. L. Wheeler, and M. Koohmaraie

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Eugenol stimulates lactate accumulation yet inhibits volatile fatty acid production and eliminates coliform bacteria in cattle and swine waste, Vincent H. Varel and D. L. Miller

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Factors Regulating Lamb Longissimus Tenderness are Affected by Age at Slaughter, E. Veiseth, S. D. Shackelford, T. L. Wheeler, and M. Koohmaraie

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Characterization of biological types of cattle (Cycle VI): Carcass, yield, and longissimus palatability traits, T. L. Wheeler, L. V. Cundiff, S. D. Shackelford, and M. Koohmaraie

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Soil quality: a review of the science and experiences in the USA, B. J. Wienhold, S. S. Andrews, and D. L. Karlen

2003

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Maternal grandsire, granddam, and sire breed effects on growth and carcass traits of crossbred cattle, E. Casas and L. V. Cundiff

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Accuracy of predicting weight and percentage of beef carcass retail product using ultrasound and live animal measures, S. P. Greiner, G. H. Rouse, D. E. Wilson, L. V. Cundiff, and T. L. Wheeler

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Prediction of retail product weight and percentage using ultrasound and carcass measurements in beef cattle, S. P. Greiner, G. H. Rouse, D. E. Wilson, L. V. Cundiff, and T. L. Wheeler

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The relationship between ultrasound measurements and carcass fat thickness and longissimus muscle area in beef cattle, S. P. Greiner, G. H. Rouse, D. E. Wilson, L. V. Cundiff, and T. L. Wheeler

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Molecular Cloning and Endometrial Expression of Porcine Amphiregulin, Jong G. Kim, Jeffrey L. Vallet, and Ronald K. Christenson

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Frequency of Luteinizing Hormone Pulses in Cattle Influences Duration of Persistence of Dominant Ovarian Follicles, Follicular Fluid Concentrations of Steroids, and Activity of Insulin-Like Growth Factor Binding Proteins, F. N. Kojima, E. G. M. Bergfeld, M. E. Wehrman, A. S. Cupp, K. E. Fike, D. V. Mariscal-Aguayo, T. Sanchez-Torres, M. Garcia-Winder, D. T. Clopton, A. J. Roberts, and J. E. Kinder

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Growth and pubertal development in Brahman-, Boran-, Tuli-, Belgian Blue-, Hereford- and Angus-sired F1 bulls, D. D. Lunstra and L. V. Cundiff

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Germ Cell Development in Meishan and White Composite Gilts, Susan A. McCoard, Thomas H. Wise, and J. Joe Ford

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Theory and application of genome-based approaches to improve the quality and value of beef, Timothy P.L. Smith, R. Mark Thallman, Eduardo Casas, Steven D. Shackelford, Tommy L. Wheeler, and Mohammad Koohmaraie

2002

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Characterization of Porcine Uterine Estrogen Sulfotransferase, J. G. Kim, J. L. Vallet, G. A. Rohrer, and R. K. Christenson

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Regulation of Endometrial Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) in the Ewe, W. J. McGuire, K. Imakawa, K. Tamura, C. S. R. Meka, and R. K. Christenson

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Genetic Parameters of Reproductive Traits in Sheep, A. Rosati, E. Mousa, L. D. Van Vleck, and L. D. Young

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Carvacrol and Thymol Reduce Swine Waste Odor and Pathogens: Stability of Oils, Vincent H. Varel

2001

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Germplasm Evaluation Program- Progress Report No. 21, Larry V. Cundiff, T. L. Wheeler, Keith Gregory, Steven D. Shackelford, M. Koohmaraie, R.M. Thallman, and L. Dale Van Vleck

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Germplasm Evaluation Program- Progress Report No. 20, Larry V. Cundiff, T. L. Wheeler, Steven D. Shackelford, M. Koohmaraie, R.M. Thallman, Keith Gregory, and L. Dale Van Vleck

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Improved antimicrobial activity of nisin-incorporated polymer films by formulation change and addition of food grade chelator, C. N. Cutter, J. L. Willett, and G. R. Siragusa

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A porcine BAC library with tenfold genome coverage: a resource for physical and genetic map integration, Scott C. Fahrenkrug, Gary A. Rohrer, Brad A. Freking, Timothy P. L. Smith, Kazutoyo Oswoegawa, Chung Li Shu, Joseph J. Catanese, and Pieter J. de Jong

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Use of Heart Girth to Predict Body Weight of Working Oxen in the Ethiopian Highlands, M. R. Goe, J. R. Alldredge, and D. Light

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A rapid twofold dilution method for microbial enumeration and resuscitation of uninjured and sublethally injured bacteria, D H. Kang and G R. Siragusa

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A Rapid Twofold Dilution Method for Microbial Enumeration and Resuscitation of Uninjured and Sublethally Injured Bacteria, D. H. Kang and G. R. Siragusa

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Characterization of Uterine Epidermal Growth Factor During Early Pregnancy in Pigs, J. G. Kim, J. L. Vallet, and R. K. Christenson

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The Role of Cholecystokinin in the Expression of Seasonal Variation in the Feed Intake and Eating Pattern of Red Deer (Cervus elaphus), S. M. Rhind, S. R. McMillen, J. C. Pekas, and E. Duff

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Insulin-Like Growth Factor Binding Proteins in the Bovine Anterior Pituitary, Andrew J. Roberts, Richard N. Funston, and Gary E. Moss

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Structure of the Genes for Porcine Endometrial Secreted and Membrane Folate Binding Proteins, J. L. Vallet, T. P. L. Smith, T. S. Sonstegard, M. Heaton, and S. C. Fahrenkrug

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Effect of Extraction Buffer on Estimating Calpain and Calpastatin Activity in Postmortem Ovine Muscle, E. Veiseth and M. Koohmaraie

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Alternative Embryo Transfer on Day 3 or Day 5 for Reducing the Risk of Multiple Gestations, Hye-Gyun Yoon, San-Hyun Yoon, Weon-Young Son, Jong Gug Kim, Kyung-Soon Im, and Jin-Ho Lim

2000

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Mutations in the genome of porcine reproductive and respiratory syndrome virus responsible for the attenuation phenotype, R. Allende, G. F. Kutish, W. Laegreid, Z. Lu, T. L. Lewis, D. L. Rock, J. Friesen, J. A. Galeota, A. R. Doster, and F. A. Osorio

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Development and Demonstration of RNA Isolation and RT−PCR Procedures to Detect Escherichia coli O157:H7 Gene Expression on Beef Carcass Surfaces, E. D. Berry

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Development and demonstration of RNA isolation and RT-PCR procedures to detect Escherichia coli O157:H7 gene expression on beef carcass surfaces, E.D. Berry

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Genetic diversity and transcriptional analysis of the bys1 gene from Blastomyces dermatitidis, James L. Bono; Basam Jaber; Mark A. Fisher; Raed O. Abuodeh; Erin O’Leary-Jepson; Gene M. Scalarone; and Laurens H. Smith, Jr.

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Germplasm Evaluation Program Progress Report No. 19, Larry V. Cundiff, Keith Gregory, T. L. Wheeler, Steven D. Shackelford, M. Koohmaraie, Harvey C. Freetly, and D.D. Lunstra

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Luteinizing Hormone Secretion as Affected by Hypophyseal Stalk Transection and Estradiol-17β in Ovariectomized Gilts, J. J. Ford, J. G. Berardinelli, R. K. Christenson, and L. L. Anderson

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Mapping quantitative trait loci for bovine ovulation rate, Brian W. Kirkpatrick, Becky M. Byla, and Keith E. Gregory

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Dehydrogenase and Oxoreductase Activities of Porcine Placental 11β-Hydroxysteroid Dehydrogenase, Harold G. Klemcke

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Identification and Characterization of a New Allele for the Beta Subunit of Follicle-Stimulating Hormone in Chinese Pig Breeds, M. D. Li, G. A. Rohrer, T. H. Wise, and J. J. Ford