Density-Gradient Centrifugation: Non-Ideal Sedimentation and the Interaction of Major and Minor Components

Authors

Myron K. Brakke, University of Nebraska-Lincoln
J. M. Daly, Nebraska Agricultural Experiment Station

Date of this Version

1965

Comments

Published in SCIENCE, New Series, Vol. 148, No. 3668 (Apr. 16, 1965), pp. 387-389.

Abstract

A small amount of southern bean mosaic virus was contained in a narrow zone after density-gradient centrifugation, but in a much wider zone after centrifugation with a large amount of a second virus. Zone-spreading of a major component by non-ideal sedimentation in density-gradient centrifugation can cause zone spreading of a minor component that the major component overlaps.