Density-Gradient Centrifugation: Non-Ideal Sedimentation and the Interaction of Major and Minor Components

Authors

Myron K. Brakke, University of Nebraska-Lincoln
J. M. Daly, Nebraska Agricultural Experiment Station

Document Type

Article

Date of this Version

1965

Comments

Published in SCIENCE, New Series, Vol. 148, No. 3668 (Apr. 16, 1965), pp. 387-389.

Abstract

A small amount of southern bean mosaic virus was contained in a narrow zone after density-gradient centrifugation, but in a much wider zone after centrifugation with a large amount of a second virus. Zone-spreading of a major component by non-ideal sedimentation in density-gradient centrifugation can cause zone spreading of a minor component that the major component overlaps.