Molecular serotyping of Escherichia coli O111:H8

Authors

Lisa M. Durso, USDA, ARS, U.S. Meat Animal Research CenterFollow
James L. Bono, USDA, ARS, U.S. Meat Animal Research CenterFollow
James E. Keen, USDA, ARS, U.S. Meat Animal Research CenterFollow

Document Type

Article

Date of this Version

4-9-2007

Comments

Published in Journal of Microbiological Methods 69 (2007) 381–383.

Abstract

AccurateE. coli serotyping is critical for pathogen diagnosis and surveillance of non-O157 Shiga-toxigenic strains, however few laboratories have this capacity. The molecular serotyping protocol described in this paper targets the somatic and flagellar antigens of E. coli O111:H8 used in traditional serotyping, and can be performed routinely in the laboratory.