U.S. Department of Agriculture: Agricultural Research Service, Lincoln, Nebraska
Date of this Version
3-2017
Citation
Published in ANNUAL REPORT OF THE BEAN IMPROVEMENT COOPERATIVE, Vol. 60, March 2017.
Abstract
INTRODUCTION- The Andean common bean landrace G19833 (Chaucha Chuga) was recently used to obtain the first reference genome of Phaseolus vulgaris (Schmutz et al, 2014). A large quantity of sequence information is available for this landrace which includes BAC libraries, cDNA libraries, SNP databases, gene expression profiles from various tissues (RNAseq), etc. Additionally, G19833 has been used to generate RIL populations for mapping traits such as phosphorous acquisition, agronomic performance, and disease resistance. G19833 has also been reported as resistant to the anthracnose, angular leaf spot, and Ascochyta blight pathogens and susceptible to the bean golden mosaic and bean common mosaic, viruses (Reviewed by Broughton et al, 2003). However, little is known about the reaction of G19833 to the bean rust pathogen. We report here the reaction of G19833 to a set of 13 races of the rust pathogen, 10 Mesoamerican and three Andean. Together, these races overcome all known rust resistance genes in common bean. Rust resistance in the under-utilized Andean beans lags behind that of Mesoamerican beans. The objective of this study was to determine the spectrum of resistance of Andean G19833 to 13 different virulent races of the bean rust pathogen.
MATERIALS AND METHODS - All disease evaluations were conducted under greenhouse conditions using published methodologies (Stavely, 1983). Landrace G19833 was inoculated with races 15-1 (41), 6-10 (44), 15-3 (47), 22-6 (49), 31-1 (53), 31-7 (58), 31-22 (67), 21-0 (72), 73 (6-15), 84 (37-1), 85 (6-31), 102 (29-0), and 108 (22-52). Rust resistance controls included Pinto 114, Aurora (Ur-3), Mexico 235 (Ur-3+), Early Gallatin (Ur-4), Mexico 309 (Ur-5), Golden Gate Wax (Ur-6), Great Northern 1140 (Ur-7), Pompadour Checa 50 (Ur-9/Ur-12), PI181996 (Ur-11), Redlands Pioneer (Ur-13), Ouro Negro (Ur-14), PI260418 (Ur-Unnamed), and PI310762 (Ur-Unnamed), and Compuesto Negro de Chimaltenango, CNC (Ur-Unnamed). Rust phenotypic evaluations were conducted twelve days after inoculations.