Molecular Serotyping of Escherichia coli O26:H11

Authors

Lisa M. Durso, USDA, ARS, U.S. Meat Animal Research Center, Clay Center, NebraskaFollow
James L. Bono, USDA, ARS, U.S. Meat Animal Research Center, Clay Center, NebraskaFollow
James E. Keen, USDA, ARS, U.S. Meat Animal Research Center, Clay Center, NebraskaFollow

Document Type

Article

Date of this Version

8-1-2005

Comments

Published in APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Aug. 2005, p. 4941–4944.

Abstract

Serotyping is the foundation of pathogenic E. coli diagnostics; however, few laboratories have this capacity. We developed a molecular serotyping protocol that targets, genetically, the same somatic and flagellar antigens of E. coli O26:H11 used in traditional serotyping. It correctly serotypes strains untypeable by traditional methods, affording primary laboratories serotyping capabilities.