Draft genome assembly of Passalora sequoiae a needle blight pathogen on Leyland cypress

Authors

Warren E. Copes, USDA-ARS National Peanut Research LaboratoryFollow
Jorge Ibarra Caballero, Colorado State UniversityFollow
Ebrahiem Babiker, USDA ARSFollow
Jane E. Stewart, Colorado State UniversityFollow
Valerie A. Orner, USDA-ARS National Peanut Research LaboratoryFollow
Alan S. Windham, University of TennesseeFollow
Renee Arias, United States Department of AgricultureFollow

ORCID IDs

Warren E. Copes http://orcid.org/0000-0001-7360-7506

Date of this Version

2020

Citation

Copes et al. BMC Res Notes (2020) 13:505

https://doi.org/10.1186/s13104-020-05328-3

Comments

This article is licensed under a Creative Commons Attribution 4.0 International License,

Abstract

Objective: Passalora sequoiae (family Mycosphaerellaceae) causes a twig blight on Leyland cypress that requires numerous fungicide applications annually to minimize economic losses for ornamental plant nursery and Christmas tree producers. The objective was to generate a high-quality draft assembly of the genome of P. sequoiae as a resource for primer development to investigate genotype diversity. Data description: We report here the genome sequence of P. sequoiae 9LC2 that was isolated from Leyland cypress ‘Leighton Green’ in 2017 in southern Mississippi, USA. The draft genome was obtained using Pacific Biosciences (PacBio) SMRT and Illumina HiSeq 2500 sequencing. Illumina reads were mapped to PacBio assembled contigs to determine base call consistency. Based on a total of 44 contigs with 722 kilobase (kb) average length (range 9.4 kb to 3.4 Mb), the whole genome size was estimated at 31,768,716 bp. Mapping of Illumina reads to PacBio contigs resulted in a 1000 × coverage and were used to confirm accuracy of the consensus sequences.