Wheat Polyphenol Oxidase: Distribution and Genetic Mapping in Three Inbred Line Populations

Authors

Tigst Demeke, Canadian Grain Commission
Craig F. Morris, USDA-ARS
Kimberly A. Garland-Campbell, USDA-ARSFollow
Garrison E. King, USDA-ARS
James A. Anderson, University of Minnesota
Hak-Gil Chang, Kyungwon University

Date of this Version

2001

Comments

Published in Crop Sci. 41:1750–1757 (2001).

Abstract

The enzyme polyphenol oxidase (PPO) has been implicated in discoloration of Asian noodles. The recombinant inbred line (RIL) populations, M6/‘Opata 85’, NY18/CC, and ND2603/‘Butte 86’ were used to investigate the distribution, chromosome location, and number of loci involved in wheat (Triticum aestivum L.) PPO. PPO activity was measured by means of the substrates L-DOPA (L-3,4-dihydroxyphenyl-alanine) and L-tyrosine. The M6/Opata 85 RIL population had a normal distribution, while the ND2603/Butte 86 RIL population had a bimodal distribution for PPO activity (L-DOPA assay).