Evaluation of a Real-Time PCR Kit for Detecting Escherichia coli O157 in Bovine Fecal Samples

Authors

James L. Bono, U.S. Meat Animal Research Center, USDA-ARS, Clay Center, NebraskaFollow
James E. Keen, U.S. Meat Animal Research Center, USDA-ARS, Clay Center, NebraskaFollow
Laura C. Miller, U.S. Meat Animal Research Center, USDA-ARS, Clay Center, NebraskaFollow
James M. Fox, U.S. Meat Animal Research Center, USDA-ARS, Clay Center, Nebraska
Carol G. Chitko-McKown, U.S. Meat Animal Research Center, USDA-ARS, Clay Center, NebraskaFollow
Michael P. Heaton, U.S. Meat Animal Research Center, USDA-ARS, Clay Center, NebraskaFollow
William Laegreid, University of Nebraska-LincolnFollow

Document Type

Article

Date of this Version

3-1-2004

Comments

Published in APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Mar. 2004, p. 1855–1857.

Abstract

A commercially available real-time, rapid PCR test was evaluated for its ability to detect E. coli O157. Both the sensitivity and specificity of the assay were 99% for isolates in pure culture. The assay detected 1 CFU of E. coli O157:H7 g^-1 in artificially inoculated bovine feces following enrichment.