U.S. Department of Agriculture: Agricultural Research Service, Lincoln, Nebraska

 

Document Type

Article

Date of this Version

3-1-2004

Comments

Published in APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Mar. 2004, p. 1855–1857.

Abstract

A commercially available real-time, rapid PCR test was evaluated for its ability to detect E. coli O157. Both the sensitivity and specificity of the assay were 99% for isolates in pure culture. The assay detected 1 CFU of E. coli O157:H7 g-1 in artificially inoculated bovine feces following enrichment.

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