USDA Agricultural Research Service --Lincoln, Nebraska
Date of this Version
March 2004
Abstract
A commercially available real-time, rapid PCR test was evaluated for its ability to detect E. coli O157. Both the sensitivity and specificity of the assay were 99% for isolates in pure culture. The assay detected 1 CFU of E. coli O157:H7 g-1 in artificially inoculated bovine feces following enrichment.
Comments
Published in APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Mar. 2004, p. 1855–1857.