Polyvinyl Alcohol-Fixative-Adhesive for Small Helminths and Protozoa

Authors

Glenn L. Hoffman, US Fish and Wildlife ServiceFollow

Date of this Version

1954

Comments

Published in Transactions of the American Microscopical Society, Vol. 73, No. 3 (Jul., 1954), pp. 328-329

Abstract

Following the method of Brooke and Goldman (1949), the author was able to fix, mount and stain small helminths and parasitic protozoa of fish in polyvinyl alcohol-fixative smears. However, because there was much granular material in the PVA-Schaudin's mixture, AFA fixative was substituted for the Schaudin's. The PVA went into solution readily using this fixative. The solution was accomplished by adding 5 grams Elvanol #71-30¹ to 62 ml. of water, heating to 950 C. until dissolved and adding 5 ml. of acetic acid, 1-1/2 ml. of glycerine, 10 ml. of formaldehyde, and 30 ml. of 95 percent alcohol.