Noninvasive Assessment of Cardiac Abnormalities in Experimental Autoimmune Myocarditis by Magnetic Resonance Microscopy Imaging in the Mouse

Authors

Chandirasegaran Massilamany, University of Nebraska-LincolnFollow
Vahid Khalilzad-Sharghi, University of Nebraska-LincolnFollow
Arunakumar Gangaplara, University of Nebraska-Lincoln
David Steffen, University of Nebraska-LincolnFollow
Shadi F. Othman, University of Nebraska - LincolnFollow
Jay Reddy, University of Nebraska-LincolnFollow

Date of this Version

2016

Document Type

Article

Citation

Journal of Visualized Experiments 88 (June 2014), e51654. doi:10.3791/51654

The video component of this article can be found at http://www.jove.com/video/51654/

Comments

Copyright © 2014 Journal of Visualized Experiments. Used by permission.

Abstract

Myocarditis is an inflammation of the myocardium, but only ~10% of those affected show clinical manifestations of the disease. To study the immune events of myocardial injuries, various mouse models of myocarditis have been widely used. This study involved experimental autoimmune myocarditis (EAM) induced with cardiac myosin heavy chain (Myhc)-α 334-352 in A/J mice; the affected animals develop lymphocytic myocarditis but with no apparent clinical signs. In this model, the utility of magnetic resonance microscopy (MRM) as a non-invasive modality to determine the cardiac structural and functional changes in animals immunized with Myhc-α 334-352 is shown. EAM and healthy mice were imaged using a 9.4 T (400 MHz) 89 mm vertical core bore scanner equipped with a 4 cm millipede radio-frequency imaging probe and 100 G/cm triple axis gradients. Cardiac images were acquired from anesthetized animals using a gradient-echo-based cine pulse sequence, and the animals were monitored by respiration and pulse oximetry. The analysis revealed an increase in the thickness of the ventricular wall in EAM mice, with a corresponding decrease in the interior diameter of ventricles, when compared with healthy mice. The data suggest that morphological and functional changes in the inflamed hearts can be non-invasively monitored by MRM in live animals. In conclusion, MRM offers an advantage of assessing the progression and regression of myocardial injuries in diseases caused by infectious agents, as well as response to therapies.