Development of Luminescent Mycobacterium avium subsp. paratuberculosis for Rapid Screening of Vaccine Candidates in Mice

Authors

Valerie Rosseels, Mycobacterial Immunology, WIV-Pasteur Institute Brussels, 642 Engelandstraat, B-1180 Brussels, Belgium
Virginie Roupie, Mycobacterial Immunology, WIV-Pasteur Institute Brussels, 642 Engelandstraat, B-1180 Brussels, Belgium
Denise K. Zinniel, University of Nebraska - LincolnFollow
Raul G. Barletta, University of Nebraska - LincolnFollow
Kris Huygen, University of Nebraska - Lincoln

Document Type

Article

Date of this Version

June 2006

Comments

Published in INFECTION AND IMMUNITY, June 2006, p. 3684–3686. Copyright © 2006, American Society for Microbiology. Used by permission.

Abstract

Mycobacterium avium subsp. paratuberculosis is a slowly growing mycobacterial species, requiring 6 to 8 weeks of culture before colonies can be counted visually. Here, we describe the development of luminescent M. avium subsp. paratuberculosis expressing luxAB genes of Vibrio harveyi and its use for vaccine testing in an experimental mouse model, replacing fastidious CFU counting by rapid luminometry.