CD8⁺ T Cells Responding to Influenza Infection Reach and Persist at Higher Numbers Than CD4⁺ T Cells Independently of Precursor Frequency

Authors

Timothy J. Powell, Trudeau Institute, Saranac Lake, NY
Deborah M. Brown, University of Nebraska-LincolnFollow
Joseph A. Hollenbaugh, Trudeau Institute, Saranac Lake, NY
Tina Charbonneau, Trudeau Institute, Saranac Lake, NY
Roslyn A. Kemp, Trudeau Institute, Saranac Lake, NY
Susan L. Swain, Trudeau Institute, Saranac Lake, NY
Richard Dutton, Trudeau Institute, Saranac Lake, NY

Document Type

Article

Date of this Version

10-2004

Comments

Published in Clinical Immunology 113:1 (October 2004), pp. 89-100; doi 10.1016/j.clim.2004.05.006 Copyright © 2004 Elsevier Inc. Used by permission. http://www.sciencedirect.com/science/journal/15216616

Abstract

The activation, localization, phenotypic changes, and function of CFSE-labeled naive influenza-specific CD8⁺ and CD4⁺ T cells following influenza infection were examined. Response of adoptively transferred CD8⁺ T cells was seen earliest in draining lymph node. Highly activated cells were found later in the lung, airways, and spleen, were cytolytic, and expressed IFN-γ upon restimulation. Similar amounts of division at early time points, but higher numbers of CD8⁺ T cells, were detected at 9 and 30 days postinfection after cotransfer of CD4⁺ and CD8⁺ T cells followed by infection. Transfer of much smaller numbers of CD4⁺ and CD8+ T cells led to more extensive expansion but the same difference in final number between the two cell types. These studies demonstrate how CD8⁺ and CD4⁺ T cells respond to influenza at early time points postinfection and the differential kinetics of antigenspecific CD4⁺ and CD8⁺ T cells.