CD8⁺ T Cells Responding to Influenza Infection Reach and Persist at Higher Numbers Than CD4⁺ T Cells Independently of Precursor Frequency

Authors

• Timothy J. Powell, Trudeau Institute, Saranac Lake, NY
• Deborah M. Brown, University of Nebraska-LincolnFollow
• Joseph A. Hollenbaugh, Trudeau Institute, Saranac Lake, NY
• Tina Charbonneau, Trudeau Institute, Saranac Lake, NY
• Roslyn A. Kemp, Trudeau Institute, Saranac Lake, NY
• Susan L. Swain, Trudeau Institute, Saranac Lake, NY
• Richard Dutton, Trudeau Institute, Saranac Lake, NY

Document Type

Article

Date of this Version

10-2004

Comments

Published in Clinical Immunology 113:1 (October 2004), pp. 89-100; doi 10.1016/j.clim.2004.05.006 Copyright © 2004 Elsevier Inc. Used by permission. http://www.sciencedirect.com/science/journal/15216616

Abstract

The activation, localization, phenotypic changes, and function of CFSE-labeled naive influenza-specific CD8⁺ and CD4⁺ T cells following influenza infection were examined. Response of adoptively transferred CD8⁺ T cells was seen earliest in draining lymph node. Highly activated cells were found later in the lung, airways, and spleen, were cytolytic, and expressed IFN-γ upon restimulation. Similar amounts of division at early time points, but higher numbers of CD8⁺ T cells, were detected at 9 and 30 days postinfection after cotransfer of CD4⁺ and CD8⁺ T cells followed by infection. Transfer of much smaller numbers of CD4⁺ and CD8+ T cells led to more extensive expansion but the same difference in final number between the two cell types. These studies demonstrate how CD8⁺ and CD4⁺ T cells respond to influenza at early time points postinfection and the differential kinetics of antigenspecific CD4⁺ and CD8⁺ T cells.