Agronomy and Horticulture Department

 

Date of this Version

1-17-1969

Comments

Published in SCIENCE, New Series, Vol. 163, No. 3864 (Jan. 17, 1969), pp. 293-294.

Abstract

Deterrent B, a compound apparently involved in the resistance of Melilotus infesta to the sweetclover weevil Sitona cylindricollis, has been isolated from leaves by a combination of preparative paper chromatography, sublimation, and crystallization. The compound has been identified as ammonium nitrate. Isolated deterrent B and ammonium nitrate have identical feeding deterrent activities. Although the deterrent principle was isolated as the ammonium salt, nitrate ion is probably responsible for the deterrent activity in vivo.

Share

COinS