Fluorometric Assay of Free and Bound, cis- and trans-o-Hydroxycinnamic Acid in a Single Plant Extract

Authors

Francis A. Haskins, University of Nebraska-LincolnFollow
H. J. Gorz

Document Type

Article

Date of this Version

10-1970

Comments

Published in CROP SCIENCE, VOL.1O, SEPTEMBER-OCTOBER 1970.

Abstract

Fluorescence is measured on unhydrolyzed and hydrolyzed plant extracts, with and without prior ultraviolet treatment. Readings permit direct determination of free o-hydroxycinnamic acid (o-HCA), free trans-o-HCA, total o-HCA, and total trans-o-HCA. Levels of bound trans-o-HCA and free and bound cis-o-HCA are calculated by appropriate subtraction.