Application of Sequence-related Amplified Polymorphism Markers for Characterization of Turfgrass Species

Authors

Hikmet Budak, University of Nebraska-LincolnFollow
Bob Shearman, University of Nebraska-LincolnFollow
Roch E. Gaussoin, University of Nebraska-LincolnFollow
Ismail M. Dweikat, University of Nebraska-LincolnFollow

Date of this Version

2004

Citation

HortScience 39(5):955-958. 2004.

Comments

Copyright 2004 American Society for Horticultural Science. Used by Permission.

Abstract

A simple marker technique called sequence-related amplified polymorphism (SRAP) provides a useful tool for estimation of genetic diversity and phenetic relationships in natural and domesticated populations. Previous studies and our initial screen show SRAP is highly polymorphic and more informative when compared to AFLP, RAPD and SSR markers. In this study, applicability of the SRAP markers to obtain an overview of genetic diversity and phenetic relationships resent among cool-season (C3) and warm-season (C4) turfgrass species and their relationship with other Gramineae species were tested. Phenetic trees based on genetic similarities (UPGMA, N-J) were consistent with known taxonomic relationships. In some cases, well-supported relationships as well as evidence by genetic reticulation could be inferred. There was widespread genetic variation among C3 and C4 turfgrass species.