Agronomy and Horticulture, Department of
ORCID IDs
Document Type
Article
Date of this Version
10-2001
Citation
Theoretical and Applied Genetics 103:5 (October 2001), pp. 710–717
doi: 10.1007/s001220100597
Abstract
Field resistance to cyst nematode (SCN) race 3 (Heterodera glycines I.) in soybean [Glycine max (L.) Merr.] cv ‘Forrest’ is conditioned by two QTLs: the underlying genes are presumed to include Rhg1 on linkage group G and Rhg4 on linkage group A2. A population of recombinant inbred lines (RILs) and two populations of near-isogenic lines (NILs) derived from a cross of Forrest × Essex were used to map the loci affecting resistance to SCN. Bulked segregant analysis, with 512 AFLP primer combinations and microsatellite markers, produced a high-density genetic map for the intervals carrying Rhg1 and Rhg4. The two QTLs involved in resistance to SCN were strongly associated with the AFLP marker EATGMCGA87 (P = 0.0001, R2 = 24.5%) on linkage group G, and the AFLP marker ECCGMAAC405 (P = 0.0001, R2 = 26.2%) on linkage group A2. Two-way analysis of variance showed epistasic interaction (P = 0.0001, R2 =16%) between the two loci controlling SCN resistance in Essex × Forrest recombinant inbred lines. Considering the two loci as qualitative genes and the resistance as female index FI < 5%, jointly the two loci explained over 98% of the resistance. The locations of the two QTLs were confirmed in the NILs populations. Therefore SCN resistance in Forrest × Essex is bigenic. High-efficiency marker-assisted selection can be performed using the markers to develop cultivars with stable resistance to SCN.
Included in
Agricultural Science Commons, Agriculture Commons, Agronomy and Crop Sciences Commons, Plant Breeding and Genetics Commons
Comments
Copyright © 2001 Springer-Verlag. Used by permission.