Quantitative trait locus analysis of saturated fatty acids in a population of recombinant inbred lines of soybean

Authors

Xianzhi Wang, South Dakota State University
Guo-Liang Jiang, South Dakota State University
Marci Green, South Dakota State University
Roy A. Scott, Crop Production and Protection, GWCC-BLTSVL, USDA/ARS, Beltsville, MD
D. L. Hyten, Soybean Genomics and Improvement Laboratory, USDA Agricultural Research Service, Beltsville, MarylandFollow
P. B. Cregan, Soybean Genomics and Improvement Laboratory, USDA Agricultural Research Service, Beltsville, MarylandFollow

ORCID IDs

D. L. Hyten

Document Type

Article

Date of this Version

2012

Citation

Mol Breeding (2012) 30:1163–1179

Abstract

Soybean [Glycine max (L.) Merr.] is an important crop which contributes approximately 58% of the world’s oilseed production. Palmitic and stearic acids are the two main saturated fatty acids in soybean oil. Different levels of saturated fatty acids are desired depending on the uses of the soybean oil. Vegetable oil low in saturated fatty acids is preferred for human consumption, while for industrial applications, soybean oil with higher levels of saturated fatty acids is more suitable. The objectives of this study were to identify quantitative trait loci (QTL) for saturated fatty acids, analyze the genetic effects of single QTL and QTL combinations, and discuss the potential of marker-assisted selection in soybean breeding for modified saturated fatty acid profiles.Apopulation of recombinant inbred lines derived from the cross of SD02-4- 59 X A02-381100 was grown in five environments and the seed samples from each environment were evaluated for fatty acid content. Genotyping of the population was performed with 516 polymorphic single nucleotide polymorphism markers and 298 polymorphic simple sequence repeat markers. Eight QTL for palmitic acid, five QTL for stearic acid and nine QTL for total saturated fatty acids were detected by composite interval mapping and/or interval mapping, with a high level of consistency or repeatability in multiple environments. Most of these QTL have not been reported previously, with the exception of qPAL-A1 which confirmed the result of a previous study. Significant QTL X QTL interactions were not detected. However, significant QTL X environment interactions were detected in most cases. Comparisons of two-locus and three-locus combinations indicated that cumulative effects of QTL were significant for both palmitic and stearic acids. QTL pyramiding by molecular marker-assisted selection would be an appropriate strategy for improvement of saturated fatty acids in soybean.