High-throughput sequencing of the DBA/2J mouse genome

Authors

Xusheng Wang, University of Tennessee Health Science Center
Richa Agarwala, National Institutes of Health
John A. Capra, University of California - San Francisco
Zugen Chen, University of California - Los Angeles
Deanna M. Church, National Institutes of Health
Daniel C. Ciobanu, University of Nebraska-LincolnFollow
Zhengsheng Li, University of Tennessee Health Science Center
Lu Lu, University of Tennessee Health Science Center
Khyobeni Mozhui, University of Tennessee Health Science Center
Megan K. Mulligan, University of Tennessee Health Science Center
Stanley F. Nelson, University of California - Los Angeles
Katherine S. Pollard, University of California - San Francisco
Williams L. Taylor, University of Tennessee Health Science CenterFollow
Donald B. Thomason, University of Tennessee Health Science Center
Robert W. Williams, University of Tennessee Health Science CenterFollow

Date of this Version

2010

Comments

Wang et al.: High-throughput sequencing of the DBA/2J mouse genome. BMC Bioinformatics 2010 11(Suppl 4):O7. Copyright © 2010 Williams et al. doi:10.1186/1471-2105-11-S4-O7

Abstract

The DBA/2J mouse is not only the oldest inbred strain, but also one of the most widely used strains. DBA/2J exhibits many unique anatomical, physiological, and behavior traits. In addition, DBA/2J is one parent of the large BXD family of recombinant inbred strains [1]. The genome of the other parent of this BXD family— C57BL/6J—has been sequenced and serves as the mouse reference genome [2]. We sequenced the genome of DBA/2J using SOLiD and Illumina high throughput short read protocols to generate a comprehensive set of ~5 million sequence variants segregating in the BXD family that ultimately cause developmental, anatomical, functional and behavioral differences among these 80+ strains.