High-throughput sequencing of the DBA/2J mouse genome

Authors

• Xusheng Wang, University of Tennessee Health Science Center
• Richa Agarwala, National Institutes of Health
• John A. Capra, University of California - San Francisco
• Zugen Chen, University of California - Los Angeles
• Deanna M. Church, National Institutes of Health
• Daniel C. Ciobanu, University of Nebraska-LincolnFollow
• Zhengsheng Li, University of Tennessee Health Science Center
• Lu Lu, University of Tennessee Health Science Center
• Khyobeni Mozhui, University of Tennessee Health Science Center
• Megan K. Mulligan, University of Tennessee Health Science Center
• Stanley F. Nelson, University of California - Los Angeles
• Katherine S. Pollard, University of California - San Francisco
• Williams L. Taylor, University of Tennessee Health Science CenterFollow
• Donald B. Thomason, University of Tennessee Health Science Center
• Robert W. Williams, University of Tennessee Health Science CenterFollow

Document Type

Article

Date of this Version

2010

Comments

Wang et al.: High-throughput sequencing of the DBA/2J mouse genome. BMC Bioinformatics 2010 11(Suppl 4):O7. Copyright © 2010 Williams et al. doi:10.1186/1471-2105-11-S4-O7

Abstract

The DBA/2J mouse is not only the oldest inbred strain, but also one of the most widely used strains. DBA/2J exhibits many unique anatomical, physiological, and behavior traits. In addition, DBA/2J is one parent of the large BXD family of recombinant inbred strains [1]. The genome of the other parent of this BXD family— C57BL/6J—has been sequenced and serves as the mouse reference genome [2]. We sequenced the genome of DBA/2J using SOLiD and Illumina high throughput short read protocols to generate a comprehensive set of ~5 million sequence variants segregating in the BXD family that ultimately cause developmental, anatomical, functional and behavioral differences among these 80+ strains.