Rapid communication: Mapping of the Ca²⁺ ATPase of fast twitch 1 skeletal muscle sarcoplasmic reticulum (ATP2A1) gene to porcine chromosome 3

Authors

Daniel C. Ciobanu, University of Nebraska-LincolnFollow
Y. Ciobanu, Iowa State University
M. F. Rothschild, Iowa State University

Date of this Version

2002

Citation

J. Anim. Sci. 2002. 80:1386–1387.

Comments

Copyright 2002 American Society of Animal Science. All rights reserved. Used by permission.

Abstract

Locus Name. Ca²⁺ ATPase of fast twitch 1 skeletal muscle sarcoplasmic reticulum.

Species. Sus scrofa.

Locus Symbol. ATP2A1.

Source of Primers. Two sets of PCR primers were designed based on human ATP2A1 sequences (Gen- Bank accession numbers U96773 and U96777) available in the GenBank database. Using pig genomic DNA as a template we used set A primers to amplify approximately 1,100 bp spanning exon 1 to exon 2 and set B primers to amplify 1,200 bp gene segment covering some of exon 8 through part of exon 9. These sequences were deposited in GenBank, accession number AY027797-AY027799.