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A clinical trial was conducted to evaluate effects of two intervention strategies on the prevalence of E. coli O157:H7 shedding by feedlot steers using 384 steers and 48 pens. Intervention strategies were a direct fed microbial or vaccination against E. coli O157:H7. No differences in performance or carcass yield were observed for direct fed microbial or vaccination treatments, compared to the control. Vaccination significantly reduced the prevalence of E. coli O157:H7. In addition, we also observed a non-significant decrease in the prevalence of E. coli O157:H7 with inclusion of the direct fed microbial.