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To describe the prevalence, incidence, and duration of fecal shedding of E. coli O157:H7, 99 feedlot steers were individually tested each week of the feeding period for presence of E. coli O157:H7 in rectal feces. E. coli O157:H7 was recovered from each animal at least once during the study. Both the incidence and mean duration of shedding peaked during the middle of the feeding period. The percentage of cattle shedding E. coli O157:H7 ranged from 1% to 80% over the course of the feeding period and was affected by both the incidence and the duration of shedding.