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Nine steers were fed finishing diets in a replicated 3x3 Latin square design to determine if dietary manipulation would alter total and acid resistant E. coli populations. Manipulating diet by limit-feeding of finishing diets did not affect total or acid-resistant E. coli populations. Altering dietary ingredients did not affect total E. coli populations; however, steers fed diets containing dry-rolled or high-moisture corn had lower acid-resistant E. coli populations. Following completion of the Latin Square, all animals were fed alfalfa hay ad libitum for five days. Switching steers to alfalfa hay lowered both total and acid-resistant E. coli populations.