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Three experiments were conducted with ruminally cannulated steers to evaluate neutral detergent insoluble protein (NDIP) content of omasal samples as an escape protein estimation technique. In experiment 1, brome hay escape protein (EP) values were determined with the NDIP technique and the omasal sampling purine technique (OSPT) with no difference in EP values. In experiment 2, smooth bromegrass EP values were estimated in June and August, 1996. In situ and OSPT estimated similar EP values, but EP estimates from NDIP were lower. Experiment 3 was conducted from May through July, 1997. OSPT estimated the highest EP values. In experiments 2 and 3, NDIP had better correlation with OSPT than the in situ technique.