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Dry matter, protein, fat, phosphorus, and sulfur were measured on 100 wet distillers grains plus solubles (WDGS) samples per ethanol plant (6 plants total) with 10 samples/day, 5 consecutive days, and 2 separate months (periods). Coefficients of variation were 1.5% to 4.5% for DM within plant. Fat in WDGS averaged 11.8% and ranged from 10.7% to 13.1% across plants, with ranges of 2 to 5 percentage units within plant. Coefficients of variation were 5% to 8% and as great as 36% within plant for sulfur. The variation in protein and phosphorus were minimal.