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Dietary sulfur level is associated with hydrogen sulfide gas (H2S) levels in the rumen. These studies quantified H2S levels at different times post feeding with or without added iron (Fe) or copper (Cu) to bind sulfur. In addition, the correlations of ruminal pH measurements to ruminal H2S gas levels were estimated. Correlations between ruminal pH and hydrogen sulfide levels were not large and Fe and Cu did not affect H2S levels.