Animal Science, Department of


Date of this Version



Published in 2012 Beef Cattle Report (2012) p. 26-27. Copyright © 2012 The Board of Regents of the University of Nebraska.


Granulosa cells express vascular endothelial growth factor A (VEGFA), and VEGFA mRNA levels increase as bovine follicles reach preovulatory status. To further evaluate the role of VEGFA isoforms in follicular development, cows were either synchronized with a modified Co- Synch protocol (CIDR) or treated with melengestrol acetate (MGA) with subsequent aspiration of the dominant follicles. Higher mRNA levels for the antiangiogenic isoform, VEGFA_164B, along with AMH and CARTPT in E2-inactive follicles suggest that these factors are markers for unhealthy, atretic follicles. In contrast, higher mRNA levels for the proangiogenic isoform, VEGFA_164, in E2-active follicles indicate that this isoform may help predict healthy ovulatory follicles.