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The relationship between ruminal degradable sulfur intake (RDSI) and ruminal hydrogen sulfide concentration ([H2S]) as well as ruminal parameters were evaluated. Steers were fed diets containing organic, inorganic, and wet distiller grains with solubles (WDGS) sources of sulfur, as well as a control diet. A laboratory procedure was developedto measure RDS of ingredients. RDSI explained 65% of [H2S] variation, whereas total sulfur intake and ruminal pH, individually, explained 29 and 12%, respectively. Availability of sulfur for ruminal reduction is more important than total sulfur in the diet.