Date of this Version
Thesis (M.S.)--University of Nebraska--Lincoln, 1958. Department of Agronomy.
A study was made of factors influencing embryo development in two interspecific hybrids within the genus Melilotus. Alcohol emasculation was studied in an attempt to reduce or eliminate self-fertilization. The most favorable results were obtained from racemes immersed in 50 or 70 per cent ethyl alcohol for 15 seconds. The alcohol appeared to have no effect on the hybrid embryos which later developed.
An examination was made of gross embryo characteristics in the M. officinalis X M. alba and M. messanensis X M. hirsuta crosses. Embryos resulting from self-fertilization could be distinguished from hybrid embryos upon excision by noting differences in embryo color, endosperm condition, and ovule wall structure. Large variations in embryo development were encountered and an attempt was made to elucidate the casual factors.
Advisor: Herman J. Gorz.