Date of this Version
Thesis (M.S.)—University of Nebraska—Lincoln, 1967. Department of Veterinary Science.
The purpose of this investigation was to determine if cells which had come in contact with virus exhibited any definite and consistent enzymatic changes prior to the appearance of morphological changes. Another objective was to study enzymatic differences of specific cell cultures which were susceptible to certain viruses and those which were nonsusceptible. Uninfected and virus-infected cells grown in culture were used to describe the quantitative activities of several enzymes during the early stages of infection. Also, the isozyme patterns of some of these enzymes were studied.
Specific enzyme activities and isozyme patterns were determined in cell-free extracts from uninfected and virus-infected cell cultures. Samples were harvested before the appearance of morphological changes in the cells. Nine enzymes were tested. Four of these were hydrolytic enzymes and 5 were enzymes of carbohydrate metabolism, 2 anaerobic, and 3 aerobic.
Primary swine kidney cell cultures were infected with Beran’s enterovirus and transmissible gastro enterovirus. A swine kidney cell line was infected with Beran's virus and pseudorabies virus. Embryonic bovine kidney cell cultures were infected with pseudorabies virus. Comparable uninfected cell cultures served as controls.
Advisor: Marvin J. Twiehaus