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Document Type

Thesis

Date of this Version

1955

Citation

Thesis (M.S.)—University of Nebraska—Lincoln, 1955. Department of Physiology.

Comments

Copyright 1955, the author. Used by permission.

Abstract

If a suitable culture medium has been devised, and a clone or pure cell line is on hand, the next step in cyto culture is to ascertain the appropriate inoculum size. This is an important factor in the survival and rate of proliferation of cells in vitro.

The present work was initiated and carried out with the following objectives in mind: to determine

  1. the maximum and minimum inocula of the strain, and to establish an optimum inoculum range;

  2. the effect of the inoculum size on the rate of proliferation;

  3. the maximum number of cells attainable in 1 ml. of nutrient medium;

  4. the effect of the inoculum size on the morphology of the cells;

  5. the effect of different concentrations of conditioned medium on inocula below the minimum, and the effect of the conditioned medium on the morphology of the cells.

A “pure strain” of normal mouse liver cells was used throughout these experiments. They were cultivated on the glass bottoms of Carrel D-3.5 pyrex glass flasks. Analysis of the results indicate that there is a minimum and a maximum limit between which proliferation of this strain of Liver cells is possible under the existing conditions. These limits were found to be between 105,000 and 1,425,000 cells per ml. Below or above these limits cell proliferation is inconsistent.

Advisor: Donald M. Pace

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