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Thesis (M.S.)—University of Nebraska—Lincoln, 1965. Department of Veterinary Science.


Copyright 1965, the author. Used by permission.


The present study was undertaken with the following objectives in mind.

1. To survey the relative abundance of some of the soluble enzymes of Ascaris second stage larva.

2. To isolate and characterize and enzyme vital for parasitic growth and development.

3. To test the role of isolated enzyme in production of functional immunity in the host.

4. To compare the isolated larval enzyme with the corresponding adult form.

Sonified aqueous extracts of Ascaris suum second stage larvae were assayed for ten different enzymes. The most abundant enzyme, inorganic pyrophosphatase, was isolated and purified by ammonium sulfate fractionation and subsequent CM cellulose chromatography.

Advisor: Connell L. Marsh