Biological Systems Engineering
Date of this Version
4-3-2012
Document Type
Article
Citation
U. S. Patent No. 8,148,516.
Abstract
The present invention provides compounds, methods and systems for sequencing nucleic acid using single molecule detection. Using labeled NPs that exhibit charge-switching behavior, single-molecule DNA sequencing in a microchannel sorting system is realized. In operation, sequencing products are detected enabling real-time sequencing as successive detectable moieties flow through a detection channel. By electrically sorting charged molecules, the cleaved product molecules are detected in isolation without interference from unincorporated NPs and without illuminating the polymerase-DNA complex.
Included in
Biochemistry, Biophysics, and Structural Biology Commons, Bioinformatics Commons, Health Information Technology Commons, Other Analytical, Diagnostic and Therapeutic Techniques and Equipment Commons, Systems and Integrative Physiology Commons
Comments
U. S. government work.