Date of this Version
THE JOURNAL OF BIOLOGICAL CHEMISTRY VOL. 289, NO. 6, pp. 3639–3651, February 7, 2014
Background: PutA from Escherichia coli is a bifunctional enzyme and transcriptional repressor in proline catabolism.
Results: Steady-state and transient kinetic data revealed a mechanism in which the two enzymatic reactions are coupled by an activation step.
Conclusion: Substrate channeling in PutA exhibits hysteretic behavior.
Significance: This is the first kinetic model of bi-enzyme activity in PutA and reveals a novel mechanism of channeling activation.