Biochemistry, Department of
Date of this Version
The Journal of Biological Chemistry, Vol. 247, No. 1 Issue of January 10, pp. 170-178, 1972
The structure of an ornithine-containing lipid from Thio-bacillus thiooxidans
has been elucidated. Methanolysis of
the lipid released methyl cis-ll,lZ-methylene-Z-hydroxyoctadecanoate.
Acid hydrolysis of the residue yielded ornithine
and a mixture of fatty acids, the major components of
which were 3-hydroxyhexadecanoic and 2-hexadecenoic
acids. Identification of the 3-hydroxy fatty acid was based
on the thin layer chromatographic mobilities of the acid, its
methyl ester, the methyl ether, and acetate derivatives of its
methyl ester, on the equivalent chain lengths of the derivatives
of the acid and the acid obtained by oxidation of the
natural acid with permanganate, and on mass spectral studies.
Similar techniques were used for the identification of 2-hexadecenoic
acid. The minor fatty acids and the 2-hexadecenoic
acid were found to be degradation products of the 3-hydroxy
Biochemistry Commons, Biotechnology Commons, Other Biochemistry, Biophysics, and Structural Biology Commons
Used by permission.